New PDF release: Biomembrane Protocols. Architecture and Function

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By Graham J.M., Higgins J.A.

This moment quantity of protocols bargains the main complete set of contemporary analytical recommendations to be had for learning the structure and serve as of membranes. It gains the appliance of biochemical, spectroscopic, and fluorimetric ways to the research of molecular topology, the dynamic features of membrane constitution, and ion delivery. Antibody know-how, research of molecules all for intracellular signaling, and receptor-ligand interactions also are coated.

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Materials 1. ). 2. Butylated hydroxytoluene (BHT). 3. 4. 4. 1% (w/v) TX-l 14 in Tris-NaC1 (approx 2 mL per sample). 5. 2% (w/v) TX-l 14 in Tris-NaCl (for reextractron of aqueous phase). 6. 06% (w/v) TX-l 14,6% (w/v) sucrose in Tris-NaCl. 7. Leupeptin: (2 mg/mL) in Tris-NaC1. Store frozen. 8. Phenylmethylsulfonyl fluoride (PMSF): 200 n&f in isopropanol. Store at room temperature. Add leupeptm and PMSF directly to all the TX-l 14 solutions immediately prior to use so final concentrations are 20 clg/rnL and 1 mM respectively.

1978) Cell surface glycoproteins of rat lymphocytes. I. Correlation of mitogemc strmulation by periodate or neurammidase and galactose oxidase with the presence of papain-sensitive glycoproteins. J. Zmmunol. 121,2181-2192. 2. , and Schauer, R (1974) New sialic acids Identification of N-glycoloyl-0-acetyl neuraminic acids and N-acetyl-Oglycoloyl neuraminic acids by improved methods for detection of N-acyl and 0-acyl groups and by gas-liquid chromatography. Eur. J. Biochem 50,71-82 3 Bordier, C.

6. Muller, W. A and Gimbrone, M. , Jr. (1986) Plasmalemmal proteins of cultured vascular endothelial cells exhibit apical-basal polarity. analysis by surface-selective iodmation. J. Cell Biol 103,2389-2402. , and Rodriguez-Boulan, E. (1989) Integral and peripheral protein composition of the apical and basolateral membrane domains in MDCK cells. J. Membr. Biol 107,277-286. 8. , and Rodriguez-Boulan, E (1990) Vectorial targeting of an endogenous apical membrane sialoglycoprotein and uvomorulin m MDCK cells.

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