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Environmental Studies

By R G Berger; et al

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G. of yoghurt, cheese, produce texturing capabilities like gel formation in yoghurt via the production of polysaccharides, and also give probiotic, and health beneficial properties, especially when specifically selected starter strains are used. Now specially selected lactic acid bacteria have been developed that make possible shelf-stable yoghurts. This is because yoghurt results from a growth association between Streptococcus thermophilus and Lactobacillus bulgaricus, but it is the Lactobacillus bulgaricus that contributes most of the flavour and creates most of the acidity, whereas Streptococcus thermophilus produces only a very mild and unaromatic product.

NHCH 3 NH 2 COOCH3 ~ ~ COOCH3 More recently discovered processes include those for sotolone which occurs in lovage, fenugreek, sherry and raw sugar. This involves a deamination of the precursor, 4-hydroxy isoleucine, that is present in fenugreek seed, into 3-keto-3methyl-4-hydroxy valeric acid, followed by cyclisation into the sotolone (4,5-dimethyl-3-hydroxy-2(5H) furanone). O _NH2 ~ O H O OH O ~ Combiningthe TechnicalPush and the BusinessPull for Natural Flavours 31 Raspberry ketone can be made from a glucoside (betuloside or rhodedendrine) present in white birch bark by treatment with ]3-glucosidase to release the phenol and then selective oxidation of the secondary group o-hydroxy group on the side chain using A.

The details of the biochemical pathway whereby the microorganism converts fatty acids into methylketones have been elucidated and have been used as the basis for a large-scale fermentation process. Commercially successful microbial processes for making methyl ketones have been used for some time by companies like Unlever and Bush Boake Allen. The way microorganisms such as Penicillium roqueforti produce methyl ketones from medium chain fatty acids is of considerable scientific interest as it resembles conventional ]3-oxidation until the final step, at which a decarboxylase produces the methyl ketone, replacing the thioesterase found in ]3-oxidation.

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