Download e-book for kindle: Electron Microscopy at Molecular Dimensions: State of the by J. P. Rosenbusch, M. Alkan, M. Regenass, A. C. Steven


By J. P. Rosenbusch, M. Alkan, M. Regenass, A. C. Steven (auth.), Priv.-Doz. Dr. Wolfgang Baumeister, Professor Dr. Wolrad Vogell (eds.)

If, ten years in the past, one were requested to touch upon the customers of peering into the fmest info of biomolecular association, so much electron microscopists might, i assume a minimum of, were really en­ thusiastic. while, throughout the early seventies, numerous teams have been luck­ ful in visualizing unmarried heavy atoms, which unquestionably was once a techni­ cal triumph, this caused the main sanguine expectancies between bi­ ologists. within the following years, besides the fact that, it all started to transpire that radiation harm may well impose barriers combating us from taking complete good thing about those interesting instrumental feasibilities. thankfully, the radiation harm nightmare did no paralyze additional actions, and it used to be particularly the paintings at the red membrane which, tremendous­ ly exploiting the redundancy stratagem, published exhilarating new views. Now, nearly 5 years later, it appeared well timed and appro­ priate to prepare a global symposium to debate and weight fresh actions and present traits in "molecular microscopy". In making plans this symposium, we chosen themes in response to our view of what's very important or will deserve extra awareness within the close to destiny. bearing in mind feedback made via the invited members, a few supplementary facets have been incorporated; as a conse­ quence, this system built a little past the scope as adum­ brated through the unique identify of this assembly (Regular 2-D Arrays of Biomacromolecules: constitution decision and Assembly). because the assembly used to be prepared, we had 3 morning classes aimed toward reflecting the "State ofthe Art".

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Extra resources for Electron Microscopy at Molecular Dimensions: State of the Art and Strategies for the Future

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Microsc. The Hague, in press 6. Engel A (1978) Molecular weight determination by scanning transmission electron microscopie. Ultramicroscopy 3: 273-281 7. Engel A (1980) Current state of biological scanning transmission electron microscopy. This volume 8. Gentner NE, Mitchel REJ (1975) Ionizing radiation-induced release of a cell surface nuclease from Mircrococcus radiodurans. Radiat Res 61: 204-215 9. Heifbe W, Baumeister W (1980) in preparation 10. Kiibler 0, Baumeister W (1978) The structure of a periodic cell wall component (HPI-Iayer) of Micrococcus radiodurans.

Methanol 1 : 1 before gel electrophoresis, only the protein band with the higher apparent molecl,llar weight is found. If the protein after its elution from the sepharose column is concentrated by dialysis against polyethyleneglycol in the presence of SOS, almost exclusively the band with the lower molecular weight appears. From this we conclude that the cell wall protein can exist in two forms, which differ in apparent molecular weight. This points is strengthened by the fact that the amino acid analysis, 32 c H.

Baumeister W, Kiibler 0 (1978) Topographic study of the cell surface of Mircrococcus radiodurans. Proc Nat Acad Sci USA 75: 5525-5528 3. Dertinger H, Jung H (1969) Molekulare Strahlenbiologie. Springer, Berlin Heidel berg New York 4. Emde B, Baumeister W (1980) in preparation 5. Emde B, Wehrli E, Baumeister W (1980) The topography of the cell wall of Micrococcus radiodurans. 7th Europ. Congr. Electr. Microsc. The Hague, in press 6. Engel A (1978) Molecular weight determination by scanning transmission electron microscopie.

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