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Diagnosis

By Arthur H Douthwaite

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End of the pipette, is exactly 200 mm. from the tip. After introducing the blood, the pipette is placed vertically in a stand, its tip being pressed well into Fig. -Westergren tubes and stand for the determination of blood sedimentation-rates. a soft piece of rubber (to prevent the blood escaping) by the pressure on its upper end of a spring cap. The time is noted when the pipette is placed in position, and after the lapse of an hour the level of the upper end of the column of red cells is read off from the graduations.

One must not mistake for poikilocytosis crenated cells (Fig. 21, p. 24) or cells that have become polygonal through mutual moulding when fixed in too close apposition with one another (Fig. 20, p. 24). 3. Anisochromia : Irregularity in staining of the cells. Normally the red cells are stained approximately the same depth of colour ; but in severe anremia there is often much irregu­ larity in the staining, some cells appearing a deep, others a very pale, pink (Fig. 23, p. 24). 4. Polychromasia : Irregularity in the colour of the cells.

24). The earliest cell is large, with bright blue-staining cytoplasm and with a large nucleus in which the chromatin structure is very finely stippled and which may contain one or more nucleoli. As the cell matures it tends to shrink in size, while the cytoplasm gradually loses its baso­ philic staining properties, and after passing through a series of intermediate violet stages, becomes frankly pink, like the fully mature red cell. The primitive nucleus also tends to get smaller, both absolutely and relative to its surrounding cytoplasm, so that it comes to occupy less and less of the cell.

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