Chao-Min Cheng, Chen-Meng Kuan, Chien-Fu Chen's In-Vitro Diagnostic Devices: Introduction to Current PDF

By Chao-Min Cheng, Chen-Meng Kuan, Chien-Fu Chen

Addressing the foundation, present prestige, and destiny improvement of point-of-care diagnostics, and helping combine wisdom and instruments from Analytical Chemistry, Bioengineering, Biomaterials, and Nanotechnology, this booklet focusses on addressing the collective and mixed wishes of and academia (including scientific faculties) to successfully behavior interdisciplinary research.

In addition to summarizing and detailing built diagnostic units, this e-book will try and indicate the potential destiny tendencies of improvement for point-of-care diagnostics utilizing either scientifically established study and useful engineering wishes with the purpose to assist newbies comprehensively comprehend the improvement of point-of-care diagnostics. This contains demonstrating a number of universal yet severe ideas and mechanisms utilized in point-of-care diagnostics that deal with sensible wishes (e.g., ailment or healthcare tracking) utilizing well-developed examples to date: 1) blood glucose meters (via electrochemistry); and, 2) being pregnant assessments (via lateral move assay).

Readers of this publication will come to totally understand the right way to advance point-of-care diagnostics units, and should be encouraged to give a contribution to a serious international reason – the improvement of cheap, powerful, and conveyable in vitro diagnostics instruments (for any function) that may be used both at domestic or in source constrained areas.

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Additional info for In-Vitro Diagnostic Devices: Introduction to Current Point-of-Care Diagnostic Devices

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The electrowetting-on-dielectric (EWOD) method is an approach in which droplet movement is controlled by electrical forces. Multiple functions such as generation, mixing, sorting, and splitting of droplets can be controlled by a network of electrodes covered with a dielectric coating. The ability to precisely control the movement of small amounts of liquid offers many potential applications in IVD diagnostics [179, 180]. Rapid immunoassay, on-chip sample extraction, and rapid PCR from whole blood samples with a handheld instrument and disposable chips can be achieved [181].

Tagged-amplicon deep sequencing (TAm-Seq) has been developed to amplify and sequence large genomic regions from as little as a single copy of circulating DNA [274, 275]. It has been found that the 5995 screened genomic bases for low-frequency mutations identify mutations present in circulating DNA as low as 2 % with sensitivity and specificity >97%. Methylated DNA is another promising biomarker in urine or blood that can be used for early detection of cancer and other genetic diseases [276, 277].

5 mm. A reaction volume of on 15 mL was required. The efficiency of these plastic devices was confirmed using novel primer sets specifically targeted to the hemagglutinin (HA) gene of influenza A/H1N1 and clinical specimens. Eighty-five human clinical swab samples were tested using real-time PCR, the results of which demonstrated 100 % sensitivity and specificity (72 positive and 13 negative cases). Another PC platform was developed that used a disposable and valveless flow cell that supported PCR coupled with microarray hybridization in the same chamber [288].

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