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By Richard Kordal, Arthur Usmani, Wai Tak Law
content material: Gene expression microarrays: a platform for discovery in organic structures / Karen Seta ... [et al.] --
houses, production, and purposes of microarrays of gel-immobilized compounds and cells on a chip / Andrei D. Mirzabekov --
AlphaScreen: a hugely delicate, nonradioactive and homogeneous assay platform for drug discovery excessive throughput screening, genomics, and lifestyles technological know-how learn purposes in miniaturized layout / Changjin Wang ... [et al.] --
Light-powered microtransponders for top multiplex-level analyses of nucleic acids / Wlodek Mandecki ... [et al.] --
DNA biosensor: immunosensor functions for anti-DNA antibody / Koji Nakano ... [et al.] --
electronic optical chemistry: a singular process for the swift fabrication of customized oligonucleotide arrays / Kevin J. Luebke ... [et al.] --
Low-density array optical chip expertise for the detection of biomolecules / Bernard H. Schneider, Frederick D. Quinn, David A. Shafer --
Randomly-ordered high-density fiber optic microsensor array sensors / Jason R. Epstein, Shannon E. Stitzel, David R. Walt.
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Additional resources for Microfabricated Sensors. Application of Optical Technology for DNA Analysis
Different strains of smallpox belonging to humans and different animals were identified and discriminated from each other by using the hybridization of viral-amplified D N A with specific microchip oligonucleotides (34). ch002 Gene Expression Analysis Microchips for expression analysis can be manufactured by tethering either proper oligonucleotides or c D N A (19) ox by simultaneously tethering both oligonucleotides and c D N A on different gel pads of the same microchips (V. Shick, unpublished results).
ACS Symposium Series; American Chemical Society: Washington, DC, 2002. ch002 Contiguous Stacking Hybridization The shorter the duplexes, the higher the discrimination between perfect and mismatched ones, and at the same time, the lower their stability. The low stability of short duplexes can be increased by contiguous stacking hybridization. For example, the melting temperature of a 5-mer duplex can be increased by 5-10°C i f it is stacked to an 8-mer duplex and forms a contiguous 13-mer duplex containing a broken phosphodiester bond in one strand (5, 26).
31. ; Mirzabekov, A . Polymorphism analysis and gene detection by minisequencing on an array of gel-immobilized primers. Nucl. Acids Res. 1999, 27, E19. 32. Strizhkov, B . N . ; Drobyshev, A . L . ; Mikhailovich, V . M . ; Mirzabekov, A . D . P C R amplification on a microarray of gel-immobilized oligonucleotides: Detection of bacterial toxin- and drug-resistant genes and their mutations. BioTechniques. 2000, 29, N4, 844-857. 33. ; Shick, V . ; Lysov, Y u . ; Mirzabekov, A . Detection of single nucleotide polymorphisms of the human mu opioid receptor gene by hybridization or single nucleotide extension.