Download PDF by Shelley D. Minteer: Microfluidic Techniques: Reviews And Protocols
By Shelley D. Minteer
Hands-on researchers assessment the rules at the back of winning miniaturization and describe the most important thoughts for miniaturizing large-scale biochemical and bioanalytical tools for microchip research. The authors hide not just the most well-liked equipment for the fabrication of microchips (photolithography, laser ablation, and gentle lithography), but additionally microfluidic innovations for such bioanalytical assays and bioprocesses as DNA research, PCR, immunoassays, and telephone reactors. Highlights comprise PCR on a microchip, microscale phone culturing, and the learn of mobile approaches on a microchip. The protocols provide step by step laboratory directions, an advent outlining the foundations in the back of the procedure, lists of the mandatory gear and reagents, and pointers on troubleshooting and warding off recognized pitfalls.
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Additional resources for Microfluidic Techniques: Reviews And Protocols
7, 78–82. Reversible Bonding of Glass Devices 39 6 Reversible, Room Temperature Bonding of Glass Devices for Microfluidics Loranelle L. Lockyear Summary This chapter describes a procedure for bonding glass microdevice substrates to their top plates by contact alone. This method results in devices that are robustly bonded but that can be separated, cleaned, and reused. For glass chips that have been used for applications involving the transport of hard particles, cells, or other biological material, reversible bonding provides a way of increasing the chip’s lifetime and utility.
The aperture is usually a spherical or rectangular opening that ranges from 10 µm to several millimeters and is used to define spatially the laser beam. The discussion here follows the simplified schematic in Fig. 1, which is based on a typical laser micromachining system. After the light is emitted from the laser (1), it passes through an aperture (2) and is steered by a mirror (3) through the focusing objective (4). Ablation occurs at the polymer surface (5), which is placed on a 2D stage. Laser Ablation for Microfluidic Devices 31 The programmable stage (Aerotech, Pittsburgh, PA) is typically 2D and in the ideal setting is integrated with the laser utilized for laser ablation.
1). 10. Carefully pick up the stack and hold it up to the light, so that the channels can be seen. Once the channel ends are lined up with the access holes in the top plate, carefully put the stack back down on the work surface. Starting at one corner of the glass, press the two pieces together across the diagonal to contact bond them. 11. If one has been very diligent in cleaning the glass, there should be no, or very few, Newton rings (rainbow-colored rings around dust particles or rough glass areas).